TP53 Mutations Promote Immunogenic Activity in Breast Cancer
Figure 6
The expression of MHC Class I genes is significantly upregulated in p53-mutant MCF-7 cells versus p53-wildtype MCF-7 cells and is regulated by the cell cycle, apoptosis, and estrogen receptor (ER) activities. (a) MHC Class I genes (HLA-A, HLA-B, HLA-C, and B2M) have significantly higher mRNA expression levels in p53-mutant MCF-7 cells than in p53-wildtype MCF-7 cells, evident by real-time quantity PCR. (b) Promotion of apoptosis increases the expression of MHC Class I genes, evident by both computational and experimental analyses. (c) Inhibition of cell cycle increases the expression of MHC Class I genes. (d) Inhibition of ER alpha increases the expression of MHC Class I genes. p53-WT: p53-wildtype; p53-mut: p53-mutant; CCND1 i: CCND1 inhibitor; ERa i: ER alpha inhibitor.