Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells

<div>ALLN induces autophagy in GOF R273H-P53 cells. (a) R273H-P53 cells were treated with 10 <i>μ</i>M ALLN for different time points and expression of LC3B-II was analyzed through immunoblotting. (b) Immunoblot analysis showing expression of LC3B-II upon 48 h of exposure to CQ, ALLN, and ALLN+CQ in R273H-P53 cells. (c) Fluorescent microscopic images of MDC fluorescence in ALLN-treated (0, 5, and 10 <i>μ</i>M; 48 h) R273H-P53 cells. The scale bar represents 100 <i>μ</i>m. (d) R273H-P53 cells were treated with ALLN for 48 h and then stained with LTG dye. Green dots representing the lysosomes were counted and represented as bar diagram. The scale bar represents 100 <i>μ</i>m.</div>

Journal of Oncology

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Figure 3: Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells 

Figure 3 | Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells 