Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells

Figure 5

ALLN-induced autophagy sensitizes R273H-P53 cells by increasing ROS levels. (a) R273H-P53 cells were exposed to ALLN, ALLN+SS, and ALLN+Rapa for 48 h. NAC (5 mM) was applied 1 h prior to treatment wherever mentioned. Fold change in ROS levels is represented through bars; untreated control was taken as arbitrary unit “1.” (b) MTT assay was performed to analyze cell viability following exposure of R273H-P53 cells to ALLN and other autophagy inducers; data is represented through bar diagram. (c) R273H-P53 cells were exposed to serum starved media or Rapa (500 nM) for 48 h. Fold change in ROS levels is represented. (d) MTT assay was performed to check cell viability following exposure of R273H-P53 cells to autophagy inducers with/without NAC. indicates significant difference compared to untreated control, while # indicates significant difference compared to ALLN-treated cells. (e)(i)/(ii)/(iii) R273H-P53 cells were treated with ALLN or ALLN plus SS for 48 h. NAC was given 1 h prior to treatment wherever mentioned. Immunoblotting was thereafter performed for LC3B-II and Atg-3. indicates significant difference compared to untreated control, while # indicates significant difference compared to ALLN-treated cells.

(a)

(b)

(c)

(d)

(e)