Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2

Figure 1

The distribution profiles of six thermo-TRPs in human melanoma cells and melanocytes. (a) Western blot analysis of TRPV1 (i), TRPV2 (ii), TRPV3 (iii), TRPV4 (iv), TRPA1 (v), and TRPM8 (vi) ion channels expression level in protein samples collected from primary epidermal melanocytes, and melanoma cells of A375, G361, A2058, and SK-MEL-3. (b) Droplet digital PCR detection of six thermo-TRPs for TRPV1 (i), TRPV2 (ii), TRPV3 (iii), TRPV4 (iv), TRPA1 (v), and TRPM8 (vi) in primary epidermal melanocytes, and melanoma cells of A375, G361, A2058, and SK-MEL-3. Total mRNA from human primary epidermal melanocytes and melanoma cells of A375, G361, A2058, and SK-MEL-3 were isolated, and digital PCR screening analysis for the indicated genes was performed. Determination of copy numbers per genome of six samples. Concentration values for indicated genes (□). Error bars represented 95% confidence intervals, NTC represented nontemplate control. β-actin was used as a positive control, and all tests were performed in at least three independent experiments.

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