IL-2 Induces Transient Arrest in the G1 Phase to Protect Cervical Cancer Cells from Entering Apoptosis

Figure 7

Effect of IL-2 on the p53, p21, and cyclin D expression in cervical cancer cells. HeLa and INBL cells were incubated in the presence or absence of 100 IU/ml of IL-2 for 24 and 48 hours. RNA was obtained, RT-PCR was performed, and the products were separated by electrophoresis in an agarose gel. The results show the bands and the expression ratio for p53 (a), p21 (b), and cyclin D (c). (d) Histogram and graph representation of p53 acetylation. Statistical analysis was performed with a Student’s t-test of paired, nonparametric data. It was performed using the statistical package Graphpad Prism 5.0. .

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