Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Figure 2
Evaluation of the anoikis resistance of acidic melanoma cells grown in agarose. (a) Representative images of acidic and nonacidic A375M6 melanoma cells allowed to growth for other 5 days on agarose-coated dishes and (b) quantification of the number and the dimension of relative colonies. (c) Representative images (left) of immunoblotting for EGFR, N-cadherin, cleaved PARP1, and β-tubulin of acidic and nonacidic A375M6 melanoma cells allowed to growth for other 5 days on agarose-coated dishes and (right) densitometry graph of protein expression. (d) Representative images of the wound healing assay on acidic and nonacidic A375M6 melanoma cells allowed to growth for other 5 days on agarose-coated dishes. Images are taken immediately after scratching (t0) and 24 hours later (24h). Percentage indicates wound closure compared to t0. (e) Representative images (left) of invasiveness of acidic and nonacidic A375M6 melanoma cells allowed to growth for other 5 days on agarose-coated dishes and quantitative analysis (right) of the number of cells that migrated through Matrigel. Each experiment was conducted in triplicate and data are expressed as mean ± SEM of at least three independent experiments. p<0.05.