UBE2C Induces Cisplatin Resistance via ZEB1/2-Dependent Upregulation of ABCG2 and ERCC1 in NSCLC Cells

Figure 2

UBE2C and ZEB1/2 were involved in DDP resistance in lung cancer cells. (a) The luciferase reporter assay indicated that the transcriptional activity of UBE2C, ZEB1, and ZEB2 was higher in A549/DDP and H1299/DDP cells than their parent cells. (b, c) RT-PCR (b) and qPCR (c) demonstrating elevated mRNA level of UBE2C, ZEB1, and ZEB2 in DDP NSCLC resistant cell lines, A549/DDP and H1299/DDP, compared with their parent cells. (d, e) Immunoblotting (d) and immunofluorescent staining (e) assay demonstrating increased protein level of UBE2C, ZEB1, and ZEB2 in DDP NSCLC resistant cell lines, A549/DDP and H1299/DDP cells. (f) Cellular morphology of DDP NSCLC resistant cell lines and their parent cells was analyzed by phase contrast microscope assay. (g) Immunohistochemical staining shows that the protein levels of UBE2C, ZEB1, and ZEB2 were higher in human DDP resistance lung cancer tissues than in nonresistance cancer tissues with anthracyclines-based neoadjuvant chemotherapy. (h) Statistical analysis of the protein level of UBE2C, ZEB1, and ZEB2 in DDP non/resistance lung cancer tissues (n=20). Results were presented as mean ± SD, and the error bars represent the SD of three independent experiments. p<0.01 versus control group.

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