High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers

Figure 1

Western blot analysis of the polyclonal anti-RTNKEASI serum in A549 cell line. SDS gel was loaded with protein extract from non-small cell lung cancer A549 cell line followed by electrophoresis and immunoblotting. The blots were incubated with the preimmune serum and the polyclonal anti-RTNKEASI serum produced in rabbits from Rockland (a) or BioGenes (b). The 55 kDa target of anti-RTNKEASI appears only in sera of animals after immunization, and it is absent in the presera from the same rabbit. These results were confirmed with both Rockland and BioGenes antibodies. A PCA was performed by incubating the corresponding blots with the polyclonal anti-RTNKEASI serum from Rockland, pretreated with or without the peptide RTNKEASI (c) and also β-actin, as endogenous control. The intensity of the band approximately at 55 kDa detected by the secondary anti-rabbit horseradish peroxidase-conjugated was reduced after the preincubation of the antibody with the peptide RTNKEASI.

(a)

(b)

(c)