High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers

Figure 4

ELISA assay for sera (a, b) ALDH1A1 and (c, d) ALDHs. Sera samples from patients with NSCLC and benign lung pathologies and healthy donors were collected and subjected to an enzyme-linked immunosorbent assay with a monoclonal antibody recognizing specifically the isoform ALDH1A1 and all ALDH isoforms, respectively. (a) and (c) show individual data points, expressed as means ± SD of 3 independent experiments, while (b) and (d) show summary statistics. Boxes and whiskers represent median and (interquartile) range. A backward stepwise linear-regression modelling was used to analyze differences in ALDH1A1 and ALDH concentrations between patients with and without lung cancer after precluding age, sex, and smoking (in pack-years) as nonsignificant independent covariables.

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