A Ploidy Increase Promotes Sensitivity of Glioma Stem Cells to Aurora Kinases Inhibition
Different sensitivity of GSCs to Danusertib exposure. (a) Cell viability was analyzed in five GSC lines by MTT assay after exposure to escalating doses of Danusertib for 24, 48, and 72 h. Results represent the means from three different experiments performed in quadruplicate. Error bars represent SEM. For statistical analysis, see Supplementary Table S1. (b) Cell proliferation was evaluated through the determination of the mitotic index after exposure to Danusertib 500 nM for 48 h. Results are reported as percentages from the means of three independent experiments. Error bars represent SEM. Chi-square test on raw data was performed: p<0,01; p<0,001. (c) Clonogenic potential was evaluated after treatment for 48 h with Danusertib 50 nM and 500 nM. Results are reported in a bar graph as percentages of cell forming colonies in the treated samples over matching controls. Results are the means of three independent experiments. Error bars represent SEM. t-test was performed on raw data: p<0,05; p<0,001.
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