Live Cell Imaging Supports a Key Role for Histone Deacetylase as a Molecular Target during Glioblastoma Malignancy Downgrade through Tumor Competence Modulation

Figure 2

HDAC activity blockade leads to tumor cell shape changes in parallel to EMT and TGF-β signaling pathway through tunneling tube formation. U87-MG cells were treated with iTGF-β (25 μM SB-431542) in the presence or absence of iHDACs (100 nM TSA). Confocal microscopy characterized type I (C′, actin-rich nanotubes) and type II nanotubes (C′, actin- and tubulin-rich nanotubes) in iHDAC-treated cells. Elongation index showed that iHDAC + iTGF-β-treated cells displayed the same elongation index as that of iHDAC-treated cells (E). Qualitative RT-PCR performed with U87-MG cells treated with TSA 100 nM for 72 hours revealed that vimentin and connexin 43 expression was suppressed upon TSA treatment. Asterisks in F indicate comparisons made within the same experimental group at 24 h. , different experiments. ; ; .