2-Methoxyestradiol and Its Combination with a Natural Compound, Ferulic Acid, Induces Melanoma Cell Death via Downregulation of Hsp60 and Hsp90

Figure 5

(a) Impact of separate and combined 24-hour treatments with 10 μM 2-ME and 1 mM FA on Hsp60 protein expression in A375 cells evaluated by western blotting. (b) Impact of separate and combined 24-hour treatments with 10 μM 2-ME and 1 mM FA on Hsp70 protein expression in A375 cells evaluated by western blotting. (c) Impact of separate and combined 24-hour treatments with 10 μM 2-ME and 1 mM FA on Hsp90 protein expression in A375 cells evaluated by western blotting. Densitometric analysis of HSP/beta-actin was performed using Quantity One 4.5.2 software. The representative images are shown.

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