LncRNA PVT1 Mediates Antiapoptosis and 5-Fluorouracil Resistance via Increasing Bcl2 Expression in Gastric Cancer
Figure 3
LncRNA PVT1 regulates apoptosis by affecting Bcl2 expression. (a, b) This shows the expression of common apoptosis related factors in PVT1 overexpressed or silenced SGC-7901 cells. Results for experiment were measured in triplicate. P < 0.05. (c) Western blot analysis showing protein levels of Bcl2, Bax, and cleaved caspase-3. β-actin was the internal control. (d) Representative IHC results of Bcl2 staining in subcutaneous tumor tissues from PVT1 overexpressed and silenced SGC-7901 cells. Results were measured in triplicate for each experiment (n=3 for each group). (e) Representative IHC results of Bcl2 staining in orthotopic tumor tissues. (f, g) GSE microarray (including GSE-14210, GSE-15459, GSE-22377, GSE-29272, GSE-51105, and GSE-62254) data were divided into three groups based on PVT1 and Bcl2 expression values; patients with both high expression of PVT1 and high expression of Bcl2 were divided into high group. Patients with both low expression of PVT1 and low expression of Bcl2 were divided into low group. The remaining patients were assigned to the middle group. Kaplan-Meier analysis was performed to compare their FPS and OS differences among the three groups.