Galectin-3 Regulates the Expression of Tumor Glycosaminoglycans and Increases the Metastatic Potential of Breast Cancer

Figure 5

Galectin-3 knockdown increases the synthesis of chondroitin sulfate A and C. (a) Immunostaining for chondroitin-4-sulfate and chondroitin-6-sulfate in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (b) Quantification of chondroitin-4-sulfate and chondroitin-6-sulfate immunostaining in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (c) Quantitative PCR for N-acetyl-galactosaminyltransferase 1 (CSGalNAcT-1) in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (d) Quantitative PCR for chondroitin polymerizing factor/chondroitin synthase 2 (Chpf) in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (e) Quantification of chondroitin-4-sulfate and chondroitin-6-sulfate immunostaining in primary tumors developed by 4T1-scramble cells and 4T1-shRNA-Gal-3 cells in Lgals3^+/+ and Lgals3^−/− mice. Data are representative of two independent experiments using 3–5 animals per experiment (e). Results are shown as means ± s.d. ; .

(a)

(b)

(c)

(d)

(e)