Galectin-3 Regulates the Expression of Tumor Glycosaminoglycans and Increases the Metastatic Potential of Breast Cancer

Figure 7

Galectin-3 regulates the mRNA levels of versican, sulfatase, sulfotransferase, and the matrix metalloproteinase 9. (a) Quantitative PCR for versican in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (b) Quantitative PCR for versican in primary tumors developed by 4T1-scramble cells and 4T1-shRNA-Gal-3 cells in Lgals3^+/+ and Lgals3^−/− mice. (c) Quantitative PCR for arylsulfatase B (ARSB) in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (d) Quantitative PCR for arylsulfatase B (ARSB) in primary tumors developed by 4T1-scramble cells and 4T1-shRNA-Gal-3 cells in Lgals3^+/+ and Lgals3^−/− mice. (e) Quantitative PCR for carbohydrate sulfotransferase 11 (CHST11) in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (f) Quantitative PCR for carbohydrate sulfotransferase 11 (CHST11) in primary tumors developed by 4T1-scramble cells and 4T1-shRNA-Gal-3 cells in Lgals3^+/+ and Lgals3^−/− mice. (g) Quantitative PCR for metalloproteinase 9 (MMP9) in 4T1-scramble cells and 4T1-shRNA-Gal-3 cells. (h) Quantitative PCR for matrix metalloproteinase 9 (MMP9) in primary tumors developed by 4T1-scramble cells and 4T1-shRNA-Gal-3 cells in Lgals3^+/+ and Lgals3^−/− mice. Data are representative of two independent experiments using 3–5 animals per experiment (b, d, f, and h). Results are shown as means ± s.d. ; ; .

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