MicroRNA-181a Inhibits Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Progression by Repressing CARD11

Figure 2

Effects of miR-181a overexpression on cell cycle, proliferation, viability, and invasiveness of ABC-DLBCL cell lines. (a) CCK-8 assay shows decreased proliferation in OCI-LY10 (left panel) and U2932 (right panel) cell lines stably expressing miR-181a than that in controls. (b) FACS plots (left panel) depicting PI stained control and miR-181a overexpressing OCI-LY10 (top) and U2932 (below) cell lines demonstrating their cell cycle (G0/G1, S, and G2/M) distribution. Right panel shows quantification demonstrating increased percent G0/G1 phase cells and reduced G2/M phase cells in miR-181a overexpressing OCI-LY10 and U2932 cell lines. (c) Flow cytometry apoptosis analysis showing AnnexinV versus PI plots of control and miR-181a overexpressing OCI-LY10 (top) and U2932 (bottom) cells. Right panel shows histograms showing percent apoptotic cells (AnnexinV⁺ PI⁺) in control (black) and miR-181a overexpressing (red) OCI-LY10 and U2932 cells. As shown, miR-181a overexpression increased apoptosis in both DLBCL cell lines. (d) Transwell matrigel analysis showing the relative number of invading cells in control (black) and miR-181a overexpressing (red) OCI-LY10 and U2932 cells. The controls were set to 1.0. As shown, miR-181a overexpression decreased invasiveness of both DLBCL cell lines. Note: results are expressed as mean ± SEM of three independent experiments; and versus control group.