PKHB1 Tumor Cell Lysate Induces Antitumor Immune System Stimulation and Tumor Regression in Syngeneic Mice with Tumoral T Lymphoblasts
Figure 1
PKHB1 induces cell death and calreticulin exposure in L5178Y-R cell line. (a) Cell death was measured by Annexin-V-allophycocyanin (Annexin-V-APC) and propidium iodide (PI) staining and graphed. Dot plots of L5178Y-R cells untreated (control) and treated with control peptide 4NGG (300 μM) or CC50 (150 μM) and CC100 (300 μM) of PKHB1 for 2 h. (b) Graph represents the means (±SD) of triplicates of three independent experiments obtained as in (a). (c) Cell death induced by PKHB1 was assessed as in (a) with cells left without pretreatment (control) or pretreated (30 minutes) with BAPTA, N-Acetyl Cystein (NAC), Q-VD-OPH (QVD), Z-VAD-FMK (Z-VAD), Spautin-1 (SP-1), or Necrostatin-1 (Nec-1). (d) Calreticulin exposure induced by 4NGG (300 μM) and PKHB1 (CC50 and CC100, 2 h) was measured using FACS in L5178Y-R cell line, and representative histograms are shown. (e) Graph represents the means (±SD) of triplicates of three independent experiments obtained as in (c).