PKHB1 Tumor Cell Lysate Induces Antitumor Immune System Stimulation and Tumor Regression in Syngeneic Mice with Tumoral T Lymphoblasts

Figure 7

Schematic representation of CD47-mediated immunogenic cell death in vitro, ex vivo, and in vivo. PKHB1 induces selective ICD in L5178Y-R cell line leading to damage-associated molecular patterns (DAMP) release. DAMPs promote dendritic cell (DC) maturation and subsequence antigen presentation and T cell activation to induce cancer cell death. Moreover, PKHB1-treated cells administrated as a therapeutic vaccine induce tumor regression in syngeneic mice bearing L5178Y-R tumors. CRT, calreticulin; HMGB1, high-mobility group box 1; HSP, heat shock protein; ICD, immunogenic cell death; TSP-1, thrombospondin-1.