PKHB1 Tumor Cell Lysate Induces Antitumor Immune System Stimulation and Tumor Regression in Syngeneic Mice with Tumoral T Lymphoblasts

Table 1

TNFα, IFNγ, IL-5, IL-4, and IL-2 cytokine release (pg/mL) in cocultures of T lymphocytes with control or pulsed DCs.

	TNFα; (pg/mL)	IFNγ; (pg/mL)	IL-5; (pg/mL)	IL-4; (pg/mL)	IL-2; (pg/mL)
DCs-Control; + T-lymphocytes	38.9; ± 14	0; ± 0	0; ± 0	0; ± 0	0.2; ± 0.2
DCs-PKHB1-TCL; + T-lymphocytes	479.6; ± 156	974.33; ± 115	0; ± 0	0; ± 0	3.5; ± 0.7

Bone marrow-derived murine DCs were left with medium alone (DCs-control) or pulsed (DCs-PKHB1-TCL) during 24h with a PKHB1-TCL. Then, DCs were cocultured during 4 days with T-lymphocytes, and the supernatants were collected to quantify TNFα, IFNγ, IL-5, IL-4, and IL-2 release, by FACS. Numbers represent the means (± SD) of triplicates of three independent experiments. p<0.01; p<0.001.