High-Dose Vitamin C Tends to Kill Colorectal Cancer with High MALAT1 Expression

Figure 1

Vitamin C induces cell apoptosis, proliferation suppression, and cycle arrest in CRC. (a) Proliferation test by CCK8 assay. The proliferation of LS174T, HT-29, and SW480 was suppressed by Vc in a dose-dependent manner. (b) The apoptosis test performed by flow cytometry. The apoptosis of LS174T and HT-29 and SW480 was induced by Vc in a dose-dependent manner. The portion of late apoptosis was significantly increased when the concentration of Vc was greater than 3 mM. It should be noted that cell lysis of LS174T and SW480 was observed when cells were treated with 5 mM Vc. (c) Cell cycle analyzed by flow cytometry. The proportion of S phase was significantly increased in LS174T, HT-29, and SW480 cells after the cells were treated with different doses of Vc. (d) Column graphs of the cell cycle calculated after three independent cell cycle experiments. In (a) and (d), and when the mean of each column was compared to the mean in the group treated with 0 mM Vc. Vc con.: concentration of Vitamin C. Each experiment was performed three times.

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