High-Dose Vitamin C Tends to Kill Colorectal Cancer with High MALAT1 Expression

Figure 4

Vitamin C selectively kills colorectal cancer with higher MALAT1 expression in vitro. (a) The relative MALAT1 expression in LS174T, HT-29, and SW480 after lentiviral transfection. Negative control (WT), MALAT1-knockdown (KD). (b) The proliferation of LS174T, HT-29, and SW480 was measured in WT and KD groups treated with 0, 0.1, 0.5, 1, 2, and 3 mM Vc for 24 h (up) and 1 mM Vc for 24, 48, and 72 h (down). (c) The apoptosis test was performed in WT and KD cells under 0 mM and 5 mM Vc. (d) The cell cycle of LS174T, HT-29, and SW480 cell lines was evaluated in the WT and KD groups treated with 0, 10, and 15 mM Vc. The portion of G1, S, and G2 was labeled on the left top of each cycle graph. (e) The portion of G1, S, and G2 was calculated after three independent tests and shown in line chart. In (a), when the expression of MALAT1 in the KD group was compared to that in the WT group. In (b), and , when the cell viability was compared between WT and KD groups at 1 and 2 mM Vc in LS174 T, at 1 mM in HT-29, and at 0.5 mM and 1 mM in SW480 ((b) up) or at 24, 48, and 72 h in LS174T, at 24 h in HT-29, and at 24 h in SW480 ((b) down). In (e) and , when the portion of S phase was compared among the 0 mM, 10 mM, and 15 mM groups.

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