Cell-Permeable Bak BH3 Peptide Induces Chemosensitization of Hematologic Malignant Cells

Figure 3

Sensitivity to chemotherapy mediated by the cell-permeable Bak BH3 peptide. Ramos and CCRF-CEM cells were incubated for 12 hrs with 20 μM of the PEKT, PET, and KT peptides, in the presence or absence of cisplatin (CDDP) 40 μM. (a) Cell viability was analyzed with MTT assays. (b) Apoptosis was quantified with the active caspase-3 assay and analyzed by flow cytometry. In both assays, the graphs represent the values of three independent assays. An ANOVA test was performed with Bonferroni post hoc analysis to establish the difference between groups.  < 0.05.

(a)

(b)