Identification of a Modified HOXB9 mRNA in Breast Cancer

Figure 1

Structure and sequence of HOXB9n and HOXB9v. (a) Sequences of genomic DNA HOXB9, mRNA HOXB9n, and mRNA HOXB9v (black and grey highlighting indicates homology between sequences). (b) Schematic diagram of HOXB9n (upper) and HOXB9v (lower) transcripts showing the exons, splicing regions, and the deleted region. In HOXB9v, a 100 bp deletion in exon 1 leads to a frameshift and a stop codon formation (TAA). (c) Protein structure of HOXB9n and HOXB9v. Transcription from the start codon (ATG) to the stop codon (TAA) results in the translation of a full-length HOXB9n protein (upper). The 100 bp deletion in HOXB9v (shown in black) leads to a frameshift from AA85 (shown in grey) and truncation of protein coding by a stop codon (TAG) at AA167, which results in HOXB9v protein without hexapeptide and homeodomain.Stop codon.

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