High Expression of COL17A1 Predicts Poor Prognosis and Promotes the Tumor Progression via NF-κB Pathway in Pancreatic Adenocarcinoma

Figure 1

Differential expression between PDAC and normal tissues. (a) Differential expression of COL17A1 in GEO (GSE62452, GSE28735, GSE62165, and GSE15471) (RMA normalized gene expression values). (b) Differential expression of COL17A1 in TCGA and GTEx database by UCSC Xena visualization tool (normalized gene level expression value by log2(TPM+0.0001)). (c) Immunochemistry assay shows the high and low expression level of COL17A1 in PDAC and negative expression in adjacent normal tissues, shown at × 200 and × 400 magnification, respectively. (d) χ2 test was chosen for evaluating the differential expression between 169 PDAC and 67 adjacent normal tissues. (e) RT-qPCR shows the COL17A1 expression in six PDAC cell lines. (f) We selected the siRNA with the best knockdown effect from siRNA1, 2, and 3 according to RT-qPCR. RMA : robust multiarray average (, , , and ).