Seroprevalence of Human Betaretrovirus Surface Protein Antibodies in Patients with Breast Cancer and Liver Disease

Figure 1

(a) The single spliced mRNA of betaretrovirus Env encodes the signal peptide, surface, and transmembrane proteins. (b) The HBRV Su construct used for mammalian expression contained the cytomegalovirus immediate early promoter, HBRV SP, and Su, a TAP tag; pCMV-Su-4c contained 4 copies of M-PMV CTE inserted in the downstream of Su-TAP in either the sense (pCMV-Su-4c) or the antisense (pCMV-Su-4cr) orientation. (c) Only the pCMV-Su-4c containing the CTE in the correct orientation produced sufficient HBRV Su protein in the cell pellet and supernatant as shown by the western blot analysis.

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