Seroprevalence of Human Betaretrovirus Surface Protein Antibodies in Patients with Breast Cancer and Liver Disease

Figure 2

(a) Schematic showing large scale HBRV Su protein purification from the supernatant of HEK293T cells using a His-tag column. (b) Coomassie blue staining and western blot analysis demonstrate the purity of the HBRV gp52 protein using anti-MMTV gp52 Su in sequential elutions. (c) Western blot confirmation of ELISA positive and negative samples demonstrates reactivity using select breast cancer, PBC, and blood donor control samples. The breast cancer serum sample used in lane 7 is positive by western blot and negative by ELISA.

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