Circ_0072088 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Cancer by Absorbing miR-377

Figure 3

circ_0072088 enhances proliferation, migration, and invasion of ESCC cells in vitro. (a) qRT-PCR was utilized to determine circ_0072088 expression in TE-13 cells after sh-circ_0072088 transfection. (b and c) Nuclear and cytoplasmic separation assay for RNA was conducted for examining the expression alterations of circ_0072088 in the nucleus and cytoplasm in TE-13 cells after sh-circ_0072088 transfection. (d) qRT-PCR was used to detect the expression level of linear ZFR in TE-13 cells after sh-circ_0072088 transfection. (e) CCK-8 assay was employed for assessing TE-13 cell proliferation at 24 h, 48 h, 72 h, and 96 h after circ_0072088 downregulation. (f) The proliferation of TE-13 cells after circ_0072088 downregulation was assessed by cell colony formation. (g) Transwell assay was employed for examining migration and invasion changes in TE-13 cells after the downregulation of circ_0072088. (h and i) qRT-PCR was utilized to measure the expression of circ_0072088 and linear ZFR in ECA109 after transfection with circ_0072088 overexpression plasmid. (j) The proliferation of ECA109 cells at 24 h, 48 h, 72 h, and 96 h after the overexpression of circ_0072088 was measured by CCK-8 assay. (k) Cell colony formation assay was utilized for examining ECA109 cell proliferation after circ_0072088 overexpression. (l) Transwell assay was adopted for assessing the roles of circ_0072088 overexpression on migration as well as invasion of ECA109 cells. , , and .

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