Journal of Oncology / 2020 / Article / Fig 3

Research Article

The DNMT1/miR-34a/FOXM1 Axis Contributes to Stemness of Liver Cancer Cells

Figure 3

Effects of DNMT1 overexpression on stem-like features of MHCC97H cells. (a) DNMT1 protein amounts in MHCC97H cells transfected with Lent-DNMT1, with β-actin as a loading control. (b) miR-34a-5p levels in MHCC97H cells transfected with Lent-DNMT1. (c) miR-34a promoter methylation in MHCC97H cells transfected with Lent-DNMT1. (d, e) Representative images of spheres and colonies in MHCC97H cells transfected with Lent-DNMT1 (left) (scale bar, 200 μm); sphere formation efficiencies and colony formation rates (right). (f) CD133 expression in MHCC97H cells transfected with DNMT1 cDNA. (g) CD44 protein expression in MHCC97H cells transfected with Lent-DNMT1. (h) Bmi1, Sox2, and Oct4 mRNA amounts in MHCC97H cells transfected with Lent-DNMT1. vs. MHCC97H cells (n = 3); vs. MHCC97H cells transfected with Lent-NC.

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