Journal of Oncology / 2020 / Article / Fig 6

Research Article

The DNMT1/miR-34a/FOXM1 Axis Contributes to Stemness of Liver Cancer Cells

Figure 6

Effects of DNMT1 overexpression combined with miR-34a mimic on stemness of MHCC97H cells. (a) DNMT1 protein amounts in MHCC97H cells overexpressing DNMT1 after transfection with miR-NC or miR-34a mimic. (b) miR-34a-5p levels in MHCC97H cells overexpressing DNMT1 upon transfection with miR-NC or miR-34a mimic. (c, d) Representative images of spheres and colonies formed by MHCC97H cells overexpressing DNMT1 upon transfection with miR-NC or miR-34a mimic (left) (scale bar, 200 μm); sphere formation efficiencies and colony formation rates (right). (e) CD133 expression in MHCC97H cells overexpressing DNMT1 upon transfection with miR-NC or miR-34a mimic. (f) CD44 protein amounts in MHCC97H cells overexpressing DNMT1 after transfection with miR-NC or miR-34a mimic. (g) Bmi1, Sox2, and Oct4 mRNA amounts in MHCC97H cells overexpressing DNMT1 upon transfection with miR-NC or miR-34a mimic. vs. MHCC97H cells transfected with miR-NC (n = 3); vs. MHCC97H cells transfected with miR-34a mimic (n = 3); vs. MHCC97H cells transduced with DNMT1 cDNA.

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