Survivin Regulates Bad Gene Expression by Binding to Its Promoter and Modulates Cell Cycle and Apoptosis in Esophageal Carcinoma Cell

Figure 5

The representative PCR results of recovery DNA fragments from ChIP experiment in KYSE450 and ECA109 cell lines. The positive results of amplified fragment in the 4^th and 5^th lanes of agarose gel indicated that there were bind sites of survivin protein in the promoter region of Bad gene. 1^st lane of agarose gel: primer blank control, the group which did not add any primers. 2^nd: negative control, the group which incubated with normal mouse IgG. 3^rd: positive control, the group which incubated with 1 µl anti-RNA polymerase II (dilution, 1 : 1,000). 4–7^th: 4 primers designed to amplify various regions of the human Bad gene promoter.

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