SLCO4A1-AS1 Facilitates the Malignant Phenotype via miR-149-5p/STAT3 Axis in Gastric Cancer Cells

Figure 3

SLCO4A1-AS1 targeted and negatively regulated miR-149-5p, which was underexpressed in gastric cancer tissues. (a) The Venn diagram was used to obtain the intersection of the prediction results of the LncBase Predicted v.2 website (the targeted miRNA of SLCO4A1-AS1, http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2/index-predicted) and the specific low-expressed miRNA in STAD (provided by the TCGA database). (b–d) SLCO4A1-AS1 bound to miR-149-5p, predicted by LncBase Predicted v.2 website and verified by dual luciferase reporter gene. (e) RT-qPCR was used to detect the expression of miR-149-5p in gastric cancer tissues. (f) The Pearson correlation coefficient was used to analyze the relationship between miR-149-5p and SLCO4A1-AS1 in gastric cancer tissues (r = −0.389 and ). Experiment was repeated three times independently. versus blank; versus adjacent tissue.

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