CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis

Figure 5

CircPTK2 binds to miR-1278 to upregulate YAP1 expression in LSCC cells. (a) RT-qPCR analysis of the expression of 5 candidate miRNAs in LSCC cells. (b) RT-qPCR analysis showed the influence of circPTK2 knockdown on miR-1278 expression. (c) RT-qPCR analysis tested the overexpression efficiency of miR-1278. (d) The predicted binding site between circPTK2 and miR-1278. (e) A luciferase reporter assay was conducted to identify the binding of circPTK2 and miR-1278. (f) The predicted binding site between YAP1 and miR-1278 was predicted from starBase. (g) A luciferase reporter assay was conducted to identify the binding of YAP1 to miR-1278. (h) RIP was performed to determine the enrichment of circPTK2, miR-1278, and YAP1 in Ago2 immunoprecipitation. (i, j) RT-qPCR and western blot analyses of YAP1 mRNA and protein levels in circPTK2-knockdown cells. (k) RT-qPCR analysis of YAP1 expression in cells transfected with the indicated plasmids..

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