CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis

Figure 7

CircPTK2 promotes LSCC progression by upregulating YAP1. (a, b) RT-qPCR and western blot analyses were used to examine the overexpression efficiency of YAP1 in SNU46 and SNU899 cells. (c, d) CCK-8 assay was conducted to assess the proliferation of SNU46 and SNU899 cells. (e, f) Flow cytometry analysis was carried out to measure the cell cycle in LSCC cells. (g–i) Western blot analysis was used to measure the levels of cell cycle markers. (j, k) The apoptosis of SNU46 and SNU899 cells was evaluated using flow cytometry analysis. (l–n) The protein levels of Bax, TP53, and Bcl-2 were tested by western blot analysis. versus the sh-NC group; versus the sh-circPTK2#1 group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)