LncRNA PTPRG-AS1 Promotes the Metastasis of Hepatocellular Carcinoma by Enhancing YWHAG

Figure 4

PTPRG-AS1 directly targeted miR-199a-3p. (a) starBase prediction of PTPRG-AS1 and miR-199a-3p sites. (b) qRT-PCR was employed to estimate the quantification of miR-199a-3p after transfection with mimics-NC, miR-199a-3p mimics, inhibitor-NC, or miR-199a-3p inhibitor. (c) Luciferase reporter analysis of the targeted binding between miR-199a-3p and PTPRG-AS1. (d) qRT‐PCR was employed to exam miR-199a-3p miRNA relative expression in HCC tumor tissue. (e) The linear correlations of PTPRG-AS1 and miR-199a-3p expression were demonstrated by Pearson analysis. (f) The relative level of miR-199a-3p in cell transfected with LV-shPTPRG-AS1 and LV-NC. (g) Overexpression of miR-199a-3p inhibited proliferation of HepG2 and PLC-PRF-5 indicated by CCK-8 assay. (h) Overexpression of miR-199a-3p attenuated invasion of HepG2 and PLC-PRF-5 detected by transwell assay. WT: wild-type. MUT: mutated. , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)