LncRNA PTPRG-AS1 Promotes the Metastasis of Hepatocellular Carcinoma by Enhancing YWHAG

Figure 5

PTPRG-AS1 enhanced cell proliferation and invasion via miR-199a-3p/YWHAG axis. (a) YWHAG was the putative target of miR-199a-3p predicted by TargetScan. (b) Luciferase reporter analysis of the targeted binding between YWHAG and miR-199a-3p. (c) qRT‐PCR was employed to depict YWHAG mRNA relative quantification in HCC tumor tissue. The linear correlations of YWHAG and miR-199a-3p expression (d) were demonstrated by Pearson’s analysis. (e) qRT‐PCR was employed to estimate the mRNA quantification of YWHAG after transfection with corresponding mimics or inhibitors. (f) qRT‐PCR was employed to estimate the mRNA quantification of YWHAG in cells transfected using LV-NC or LV-shPTPRG-AS1. (g) qRT‐PCR was used to detect YWHAG mRNA relative expression after transfection with siRNA-NC and siRNA YWHAG in cells. (h) Downregulation of YWHAG inhibited cell proliferation of HepG2 and PLC-PRF-5 cells detected by CCK-8 assay. (i) Downregulation of YWHAG inhibited cell invasion of HepG2 and PLC-PRF-5 cells detected by transwell assay. WT: wild-type. MUT: mutated. , .

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