circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Figure 3

miR-141-3p is the target protein of circ_0075943. (a) The predicted binding site between circ_0075943 and miR-141-3p by Starbase (https://starbase.sysu.edu.cn/). (b) Detection of miR-141-3p mimics efficiency by real-time PCR. (c and d) The interaction between circ_0075943 and miR-141-3p was determined by dual-luciferase reporter gene checked. (e, f) The interaction between circ_0075943 and miR-141-3p was determined by the RIP test. (g) Real-time PCR was adopted to check the miR-141-3p level in tumor specimens and normal specimens. miR-141-3p was low expressed in tumors. (h) Real-time PCR was adopted to check the miR-141-3p level in BC cells. (i) The influence on exhaustion of circ_0075943 gene on the miR-141-3p level was checked by real-time PCR. .

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