circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Figure 5

circ_0075943 dominates AK2 level by targeting miR-141-3p. (a) The binding site of AK2 3′UTR and miR-141-3p is provided by TargetScan (http://www.targetscan.org/vert_71/). (b, c) The interaction between AK2 and miR-141-3p was affirmed by the dual-luciferase reporter gene. (d) AK2 level in the normal specimen and BC tumor specimen from the GEPIA database (http://gepia.cancer-pku.cn/). (e, f) Detect AK2 level in our tissue samples by real-time PCR and immunoblotting technique. (g) The immunoblotting technique was adopted to check AK2 protein level in MCF-10A, BC cells. (h) The immunoblotting technique was adopted to check the influence on miR-141-3p redevelopment on AK2 level protein. (i) Immunoblotting technique checked AK2 protein in BC cells. .

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