Research Article
HOTAIR Contributes to Stemness Acquisition of Cervical Cancer through Regulating miR-203 Interaction with ZEB1 on Epithelial-Mesenchymal Transition
Figure 1
Cisplatin-resistant cells and serum-free cultured cells from cervical cancer cell lines exhibit stem-like properties. (a–f) Detection of the stem cell markers NANOG, OCT4, CD44, ALDH1, CD133, and SOX2 in HeLa cells cultured in a serum-free medium by qRT-PCR. (g–l) Detection of the stem cell markers NANOG, OCT4, CD44, ALDH1, CD133, and SOX2 in cisplatin-resistant HeLa cells by qRT-PCR. (m) Western blot analysis of the stem cell markers NANOG, OCT4, CD133, and SOX2 in cisplatin-resistant HeLa cells. (n) Statistic analysis relative destiny of (m). values represent significant difference based on Student’s t-test from three replicates (, ).
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |
(j) |
(k) |
(l) |
(m) |
(n) |