HOTAIR Contributes to Stemness Acquisition of Cervical Cancer through Regulating miR-203 Interaction with ZEB1 on Epithelial-Mesenchymal Transition

Figure 5

miR-203 downregulated the expressions of stem markers of cervical cancer. (a, b) Cancer stem cells of HeLa enriched by treatment with cisplatin (a) or culture in a serum-free medium (b) showed the downregulated expression of miR-203. (c–h) Expression changes of the stem markers NANOG, OCT4, CD44, ALDH1, CD133, and SOX2 in cancer stem cells of HeLa along with miR-203 alteration were detected and semiquantified by qRT-PCR. NC, negative control. (i). Western blot analysis of the stem cells markers NANOG, OCT4, CD133, and SOX2 in cisplatin-resistant HeLa cells along with miR-203 alteration. (g). Statistic analysis relative destiny of (i). values represent significant difference based on Student’s t-test from three replicates (, ). HOTAIR interacted with miR-203 to post-transcriptionally regulate the expressions of ZEB1.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)