The Integrative Analysis of Thrombospondin Family Genes in Pan-Cancer Reveals that THBS2 Facilitates Gastrointestinal Cancer Metastasis

Figure 5

THBS2 promotes cancer cell EMT and metastasis. (a) LOVO or BGC-823 cells were transfected with the indicated siRNAs. Then, a western blot was performed to evaluate the THBS2 protein level. (b) The cells were transfected with indicated siRNAs. Then, a transwell migration assay was carried out to assess cell migration. (c) The transwell migration assay in (b) was summarized . (d-e) Cells were transfected with the indicated siRNAs. A wound-healing assay was performed to analyze cancer cell migration . (f) LOVO cells were transfected with indicated siRNAs. Then, total RNAs were extracted, and RT-qPCR was performed for the indicated genes . (g) BGC-823 cells were transfected with indicated siRNAs. Then, total RNAs were extracted, and RT-qPCR was performed for the indicated genes . (h–m) The correlation between THBS2 expression and the expression level of critical EMT marker genes in colon, gastric, and pancreatic cancers.

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