Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation

Figure 3

ABZ treatment inhibits the metastasis of melanoma cells by reversing the EMT process. (a-b) The relative expression levels of N-cadherin, E-cadherin, Vimentin, Occludin, and FN1 between the ABZ-treated (0.4 μM) and control groups were measured by RT-qPCR, with β-actin as the reference gene. (c-d) The expression levels of key EMT proteins were detected by western blotting. The histogram shows the relative density of N-cad, E-cad, Vimentin, Occludin, and FN1. (e) The localization and expression levels of N-cad and E-cad in mouse lung cancer tissues were determined using an immunohistochemical (IHC) assay. Image showing the IHC staining specimens (100×; left). The black dotted box is enlarged to the area corresponding to the image (right). Scale bars = 100 and 20 μm. Each point in each group in the statistical graph is calculated as the average score for five selected areas in one lung slice and represents an IHC index of one mouse. The data are expressed as means ± SD. The RT-qPCR and western blot experiments were performed thrice. ; ; .