Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation

Figure 6

Overexpression of GSK-3β/Y216F could not promote the degradation of Snail after ABZ treatment. (a) Control, GSK-3β/WT, and GSK-3β/Y216F A375 cells were treated with 0.4 μM ABZ for 24 h; western blot analysis (left) was used to detect the expression levels of AKT, pGSK-3β /Tyr216, Snail, N-cadherin, and E-cadherin in the cytoplasm. The histograms (right) show the relative density of the detected proteins. (b) The wound healing area and relative wound closure rate (%) in A375 were quantified and analyzed 24 h after treatment with 0.4 μM ABZ. The histograms (right) show the relative wound closure rate for each group. (c) Results of the transwell invasion experiments for A375 cells under different conditions. The histograms (right) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ; ; ; ns, not significant.

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