EFNB1 Acts as a Novel Prognosis Marker in Glioblastoma through Bioinformatics Methods and Experimental Validation

Figure 10

EFNB1 knockdown inhibited GBM proliferation and migration. (a, b) EFNB1 expression levels in the GBM cell lines were determined by qRT-PCR and western blot. (c, d) Silencing efficiency of EFNB1 in U251 cell. CCK-8 assays (e), clonogenic survival assay (f), and migration assay (g) were utilized to measure the rate after EFNB1 knockdown (scale bar = 100μm). (h) GSEA analysis showed the Wnt/β-catenin pathway was enriched after EFNB1 overexpression. (i) Western blot was performed to detect the expression of E-cadherin, N-cadherin, β-catenin, and GAPDH in U251 cells with or without si-EFNB1 (; ; ).

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