Chidamide and Radiotherapy Synergistically Induce Cell Apoptosis and Suppress Tumor Growth and Cancer Stemness by Regulating the MiR-375-EIF4G3 Axis in Lung Squamous Cell Carcinomas

Figure 4

Suppression of miR-375 reversed chidamide and chidamide plus radiation combinational treatment-induced apoptosis in NCI-H2170 and NCI-H226 cells. (a) Real-time PCR to determine the expression of miR-375 in control mimic and miR-375 mimic-transfected NCI-H2170 and NCI-H226 cells with radiation (2 Gy), chidamide (300 nM), and their combinational treatment, respectively. (b) CCK8 assay to evaluate the cell viability of NCI-H2170 and NCI-H226 cells with indicated treatment. (c–j) Annexin V-PI double staining to determine the apoptosis rate of NCI-H2170 and NCI-H226 cells with indicated treatment. (k) The expression of proapoptotic protein BAX and antiapoptotic protein BCL2 in NCI-H2170 and NCI-H226 cells with indicated treatment, determined by western blot analysis. All the data were statistically analyzed by Student’s t-test. ; ; and .

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