Chidamide and Radiotherapy Synergistically Induce Cell Apoptosis and Suppress Tumor Growth and Cancer Stemness by Regulating the MiR-375-EIF4G3 Axis in Lung Squamous Cell Carcinomas

Figure 6

miR-375 inhibition rescued chidamide and chidamide plus radiation combinational treatment-induced tumor growth arrest in NCI-H2170 and NCI-H226 cells, respectively. (a–c) Chidamide, radiation, and their combinational treatment inhibited tumor growth in NCI-H2170 cells. Tumor growth curves and tumor weight analysis are shown in Figures 3(b) and 3(c), respectively. (d–f) Suppression of miR-375 reversed chidamide and chidamide plus radiation combinational treatment-induced tumor growth arrest in NCI-H2170 and NCI-H226 cells, respectively. Tumor growth curves and tumor weight analysis are shown in Figures 3(e) and 3(f), respectively. (g-h) TUNEL staining to demonstrate the cell apoptosis rate in indicated xenograft tissues. (i-j) The relative intensity of TUNEL staining in (g) and (h). Scale bars: 100 μm. All the data were statistically analyzed by Student’s t-test. ; .

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