Research Article
[Retracted] Yap-Hippo Signaling Activates Mitochondrial Protection and Sustains Breast Cancer Viability under Hypoxic Stress
Figure 1
Yap knockdown induces breast cancer cell death and proliferation arrest under hypoxia stress. (a) MTT assay for cell viability in MCF-7 cells. siRNA against Yap (si-Yap) was transfected into cell before 24–48 hours hypoxia. (b) TUNEL staining was used to observe the cell apoptotic rate. siRNA against Yap (si-Yap) was transfected into cell before 24–48 hours hypoxia. (c) Caspase-3 activity was measured through ELISA. (d) qPCR was used to determine the efficiency of knockdown after si-Yap transfection. (e) CCK-8 assay was used to observe the cell proliferation capacity in MCF-7 cells under hypoxia. (f, g) qPCR assay was used to detect the transcription of VEGF and p21 in MCF-7 cells transfected with si-Yap. .
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