Yap-Hippo Signaling Activates Mitochondrial Protection and Sustains Breast Cancer Viability under Hypoxic Stress
Yap knockdown reduces mitochondrial function in hypoxia-treated breast cancer. (a) ATP production was measured through ELISA. (b, c) Mitochondrial membrane potential was determined in MCF-7 cells. siRNA against Yap (si-Yap) was transfected into cells before 24 hours hypoxia. (d, e) ROS production was detected in response to Yap knockdown. (f) Mitophagy activity was determined through the mt-Kemia assay. (g, h) qPCR assay was used to detect the transcription of Mfn2 and Opa1 in MCF-7 cells transfected with si-Yap. .
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