Journal of Oncology / 2021 / Article / Fig 5

Research Article

Yap-Hippo Signaling Activates Mitochondrial Protection and Sustains Breast Cancer Viability under Hypoxic Stress

Figure 5

F-actin/lamellipodium signaling pathway is impaired by Yap knockdown under hypoxia. (a–c) Immunofluorescence for lamellipodium. The average length and number of lamellipodia was determined. siRNA against Yap (si-Yap) was transfected into cells before 24 hours hypoxia. HIF1 adenovirus (ad-HIF1) was transfected into Yap-deleted MCF-7 cells before 24 hours hypoxia. (d) qPCR was used to detect the alterations of F-actin in response to hypoxia, si-Yap transfection, and ad-HIF1 infection. (e) Transwell assay was used to observe the migratory response of MCF-7 cells. .

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