Detection of MSH2 Gene Methylation in Extramammary Paget’s Disease by Methylation-Sensitive High-Resolution Melting Analysis

Figure 1

Using the MS-HRM standard curve to detect the methylation level of the MSH2 gene promoter. Standard curves are shown, from top to bottom: 100%, 50%, 30%, 10%, 5%, 1%, and 0% of standard methylated DNA. The lines in black and grey are two example results of EMPD samples.