Metformin Increases Sensitivity of Melanoma Cells to Cisplatin by Blocking Exosomal-Mediated miR-34a Secretion

Figure 3

Exosome secretion blockade sensitizes A375 cells to cisplatin. qPCR of miR-34a expression in A375 cells. Cells were treated with exosome secretion inhibitor GW4869, followed by control or cisplatin treatment. (b) Survival of melanoma cell (A375 cells) (CCK-8 assay). Cells were treated with exosome secretion inhibitor GW4869, followed by control or cisplatin treatment. (two-way ANOVA). (c) Western blotting of cleaved caspase-3 in A375 cells. Cells treated as abovementioned were harvested at indicated time points. β-Actin served as internal reference. Data are representatives of 3 independent experiments.

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